Understanding how prolyl-4-hydroxylase structure steers a ferryl ox-idant toward scission of a strong C-H bond.

摘要

Prolyl-4-hydroxylase (P4H) is a nonheme iron hydroxylase that regio- and stereospecifically hydroxylates proline residues in a peptide chain into R-4-hydroxyproline, which is essential for collagen crosslinking purposes in the human body. Surprisingly, in P4H a strong aliphatic C–H bond is activated, while thermodynamically much weaker aliphatic C–H groups, i.e. at the C and C positions, are untouched. Little is known on the origins of the high regio- and stereoselectivity of P4H and many nonheme and heme enzymes in general and insight into this matter may be relevant to Biotechnology as well as Drug Development. The active site of the protein contains two aromatic residues (Tyr and Trp) we expected to be crucial for guiding the regioselectivity of the reaction. We performed a detailed quantum mechanics/molecular me-chanics (QM/MM) and molecular dynamics (MD) study on wild-type and mutant structures. The work shows that, Trp243 is involved in key protein loop-loop interactions that affect the shape and size of the substrate binding pocket and its mutation has major long-range effects. By contrast, the Tyr residue guides the regio- and stereoselectivity by holding the substrate and ferryl oxidant in a specific orientation through hydrogen bonding and -stacking interactions. Compelling evidence is found that the Tyr residue is involved in expelling the product from the binding pocket after the reaction is complete. It is shown that mutations where the hydrogen bonding network that involves the Tyr and Trp residues is disrupted leads to major changes in folding of the protein and the size and shape of the substrate binding pocket. Specifically, the Trp resi-due positions the amino acid side chains of Arg and Glu in specific orientations with substrate. As such, the P4H en-zyme is a carefully designed protein with a subtle and rigid secondary structure that enables the binding of substrate, guides the regioselectivity and expels product efficiently.